What do we do?
The Plesa lab focuses on accelerating the pace at which we understand and engineer biological protein-based systems. Our primary interest is in scaling. Developing technologies which can address biological questions at scales of several order of magnitude more than existing approaches. Towards this end, we develop new technologies for gene synthesis, multiplex functional assays, in-vivo mutagenesis, and genotype-phenotype linkages for a number of different research areas and applications. These allow us to both access the huge sequence diversity present in natural systems as well as carry out testing of rationally designed hypotheses encoded onto DNA at much larger scales than previously possible.
Latest Publications
Exploring Antibiotic Resistance in Diverse Homologs of the Dihydrofolate Reductase Protein Family through Broad Mutational Scanning
Preprint - Supplementary info - Code
Preprint - Supplementary info - Code
Degenerate DropSynth for Simultaneous Assembly of Diverse Gene Libraries and Local Designed Mutants
An new version of the DropSynth multiplex gene synthesis protocol capable of assembling up to eight variants per barcoded droplet with lengths of 1 kbp.
Project - Publication - Supplementary Info
An new version of the DropSynth multiplex gene synthesis protocol capable of assembling up to eight variants per barcoded droplet with lengths of 1 kbp.
Project - Publication - Supplementary Info
