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Large Scale Protein Engineering and Characterization

​What do we do?

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The Plesa lab focuses on accelerating the pace at which we understand and engineer biological protein-based systems. Our primary interest is in scaling. Developing technologies which can address biological questions at scales of several order of magnitude more than existing approaches. Towards this end, we develop new technologies for gene synthesis, multiplex functional assays, in-vivo mutagenesis, and genotype-phenotype linkages for a number of different research areas and applications. These allow us to both access the huge sequence diversity present in natural systems as well as carry out testing of rationally designed hypotheses encoded onto DNA at much larger scales than previously possible.

Learn more about our research

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Latest Publications

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DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
​An optimized version of the DropSynth multiplex gene synthesis protocol capable of assembling up to 1536 genes at once at low cost with even more perfect assemblies.

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